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Effects of Jak-2 overexpression and high glucose on Stat-3 phosphorylation in Jak-2 mesangial cells. A: Jak-2 (130 kDa), total Stat-3 (79 kDa), phosphorylated Stat-3 (79 kDa), and β-tubulin (50 kDa) immunoblots of murine mesangial cells transfected with a Jak-2 prk-5 plasmid or a control vector (n = 3 per group). B: Phosphorylated Stat-3 levels normalized to total Stat-3 levels.
