. 2008 Aug 7;7:25. doi: 10.1186/1475-2859-7-25

Table 4.

Examples for one step enzymatic reactions employing the designer yeast made by Stewart and coworkers [88,167-170], namely Saccharomyces cerevisiae overexpressing the Acinetobacter sp. NCIB 9871 cyclohexanone monooxygenase. The data is chronologically ordered.

Entry	Substrate	Product	Substrate/Product Code	Performance: yield (ee)				Ref.
1			a: R = Me	a: 83% (≥ 98%)				[167,168]
			b: R = Et	b: 74% (≥ 98%)
			c: R = Prⁱ	c: 60% (≥ 98%)
			d: R = Pr	d: 63% (92%)
			e: R = allyl	e: 62% (95%)

					1		2

2			a: R = Me	a:	50% (49%)		---^I	[168,169]
			b: R = Et	b:	79% (95%)		69% (≥ 98%)
			c: R = Prⁱ	c:	41% (≥ 98%)		46% (96%)
			d: R = Pr	d:	54% (97%)		66% (92%)
			e: R = allyl	e:	59% (≥ 98%)		58% (≥ 98%)
			f. R = n-butyl	f:	59% (≥ 98%)		64% (98%)

					1	2	3

3			a: R = Me	a:	71% (≥ 98%)	60 % (≥ 98 %)	---^I	[168]
			b: R = Et	b:	18% (70%)	20% (70%)	---^I
			c: R = Prⁱ	c:	N.R.^II	---^I	N.R.^II
			d: R = Pr	d:	11% (≥ 98%)	---^I	8% (83%)
			e: R = allyl	e:	15% (97%)	---^I	9.3% (≥ 98%)
			f. R = n-butyl	f:	37% (56%)	---^I	11% (84%)

					1		2

4			a: R = n-Bu	a:	18% (≥ 98%)		32% (≥ 98%)	[170]
			b: R = n-Hex	b:	32% (≥ 98%)		42% (≥ 98%)
			c: R = n-Oct	c:	25% (≥ 98%)		14% (≥ 98%)
			d: R = n-	d:	39% (≥ 98%)		37% (≥ 98%)
			C₁₁H₂₃

					1^III	2^III	3^III

5			a: R = n-Pr	a:	27% (13%)	-^IV(33%)	-^IV(60%)	[170]
			b: R = n-Hex		overall yield: 44%; ratio 2 : 3 = 83 : 17

				b:	54% (29%)	(60%)	-^IV
					overall yield: 20%; ratio 2 : 3 = > 99 : < 1

6			a: R = Ph	a: 95% (> 99%)				[88]
			b: R = Bu^t	b: 47% (99%)
			c: R = Buⁿ	c: 53% (74%)

7			a: R₁= H R₂= H	1a: 18% (90%) (2a: 19% yield)				[88]
			b: R₁= H R₂= Ph	1b: 30% (30%) (no sulfone 2b detected)

8			a: R₁= H R₂= H	1a: 20% (75%) [2a: 45% yield]				[88]
			b: R₁= H R₂= CH₃	1b: 16% (20%) [2b: 15% yield 76% ee]
			c: R₁= H R₂= Ph	1c: 74% (20%) [no sulfone 2c detected]

9			R₁= CH₃	1: 84% (48%)				[88]
			R₂= CH₃	2: 10%