Figure 3. Comparative effects of BMAP-27 and BMAP-18 on growth of mammalian and insect cell lines.

Inhibition assays using alamarBlue (as described in Materials and Methods) were performed on a variety of cell lines grown in vitro. A range of concentrations of peptides was tested against all cell lines. The concentrations of peptides required for 50% inhibition (MIC₅₀) are shown. A representative parasite, T. b. brucei 427.01 PCF, treated under the same conditions, is included to indicate the marked difference in peptide concentrations required for inhibition of parasite and mammalian cell lines. Panel A: Mammalian cell lines. Panel B: Insect cell lines. Black bars: BMAP-27; Gray bars: BMAP-18. The results shown are representative of three individual experiments performed over a one year period.