Figure 6. Inhibition of LPS-induced TNF-α secretion from human PBMC by BMAP-27 and BMAP-18.

PBMC were incubated with 5 µg/mL of BMAP-27 or BMAP-18 for 30 minutes before treating with highly purified Pseudomonas aeruginosa lipopolysaccharide (10 ng/mL). After 4 hours, TNF-α was measured in the supernatants by ELISA. Gray bar: PBMC incubated with LPS alone. Black bars: PBMC incubated with BMAP-27 or with BMAP-18. The asterisks indicate the responses after treatment with LPS (p<0.01 comparing LPS alone to LPS+peptide). Three complete biological replicates were performed and the standard errors are shown.