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. Author manuscript; available in PMC: 2009 Jan 20.
Published in final edited form as: Electrophoresis. 2008 Aug;29(15):3095–3106. doi: 10.1002/elps.200800109

Figure 3.

Figure 3

Electropherograms of (A) intact HA and (B) HA treated with hyaluronidase. CE was performed on a Beckman HPCE instrument (P/ACE system 5000) equipped with a UV detector set at 200 nm. Separation and analysis by EKC with SDS were carried out on an uncoated fused-silica capillary tube (50 μm id, 85 cm total length and 65 cm from the injection point to the detector) at 25°C. The operating buffer was constituted of disodium hydrogen phosphate (40 mM), sodium tetraborate (10 mM), and SDS (40 mM) buffered at pH 9.0. HA oligomers given above the peaks.