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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Free Radic Biol Med. 2008 Jul 27;45(8):1178–1189. doi: 10.1016/j.freeradbiomed.2008.07.015

Fig. 7.

Fig. 7

Decreasing Prx II increases glioma cell cycle doubling times and alters cell cycle distribution. Cell cycle times were determined using doubling time assays and found to be longer in C6 shPrx2.3 and 36B10 shPrx2.2 cells when compared to their shNeg controls. Knocking down Prx II expression alters cell cycle distribution (A). Cell cycle distribution was analyzed by flow cytometry of propidium iodide stained nuclei and displayed as representative histograms of C6 and 36B10 stable cell lines expressing shNeg and shPrx2 (B–C). As an indicator of cell cycle distribution, activation of Cdc2 in 36B10 cells was assessed by western blot analysis detecting phosphorylation of Cdc2 on Thr 161 (D). Significance was determined using the student’s t-test where p < 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***)