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. Author manuscript; available in PMC: 2009 Jan 20.
Published in final edited form as: Dev Cell. 2007 Aug;13(2):203–213. doi: 10.1016/j.devcel.2007.07.004

Figure 1. HsSAS-6 Is Required for Procentriole Formation.

Figure 1

(A—D) HeLa cells transfected with control siRNAs or siRNAs directed against HsSAS-6, stained with antibodies against centrin-3 (red) and α-tubulin (green); DNA is shown in blue. Insets are magnified 5-fold. Scale bar, 10 μm. Cells were classified into the following categories: two pairs of centrin foci, bipolar spindle (A); two single centrin foci, bipolar spindle (B); one single centrin focus, bipolar spindle (C); one single centrin focus, monopolar spindle (D).

(E) Distribution of cells in the categories described above (n = 50 and n = 100 for 48 hr and 72 hr time points, respectively); Other: cells that could not be assigned to any of the four categories (∼5% of cells contained more than four centrin foci, for each experimental condition, and some cells contained a pair of juxtaposed centrin foci in the case of cells targeted with HsSAS-6 siRNAs). Note that monopolar spindle assembly was also observed with an independent siRNA (Leidel et al., 2005).

(F) HeLa cells expressing centrin-GFP were treated with siRNA directed against HsSAS-6 and observed 72 hr after transfection. A cell with a monopolar spindle and single centrin focus (arrow) was first imaged by DIC and fluorescence microscopy (scale bar, 10 μm), then fixed and analyzed by serial section EM (1—6: selected sections spanning the entire centriole; scale bar, 500 nm).