Skip to main content
. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2008 Sep 23;45(12):1653–1662. doi: 10.1016/j.freeradbiomed.2008.09.011

Figure 3. Activation of resting CFTR Cl- currents by pyocyanin and H2O2 in CFTR-corrected but not CF human bronchial epithelial cells (CFBE41o-).

Figure 3

(A) Activation of Cl- currents (ICl) by pyocyanin (100 μM) in CFTR-corrected CF monolayers (wtCFTR) and inhibition of pyoycanin-stimulated ICl by the CFTR blocker GlyH101 (20 μM). (B) Pyocyanin had no effect on ICl in CF monolayers homozygous for ΔF508-CFTR (ΔF508-CFTR) whereas ATP elicited a chloride secretory response similar to wtCFTR-expressing cells. (C,D) Corresponding activation of resting ICl by H2O2 (100 μM) in CFTR corrected but not CF monolayers, and inhibition of H2O2-stimulated ICl by the CFTR blocker GlyH101 (20 μM). Note that both oxidants stimulated the CFTR-mediated but not the calcium-activated Cl- conductance. (E) Summary of stimulatory effects of pyocyanin (100 μM), H2O2 (100 μM), ATP (500 μM), and forskolin (20 μM) on ICl. Data are mean values ± SE (n = 8-34 experiments), * denotes significantly different from wtCFTR, p<0.05. Measurements were done with ~1 Mio cells in 5 ml saline solution.