Table 1. Data-collection and processing statistics of the human cytoplasmatic tRNAGly acceptor-stem microhelix isoacceptor derived from the gene sequence with tRNA Database ID DG9990 (Sprinzl & Vassilenko, 2005 ▶).
Values in parentheses are for the highest resolution shell.
| Beamline | Diamond, I04 |
| Wavelength (Å) | 0.9696 |
| Space group | C2 |
| Unit-cell parameters (Å, °) | a = 37.32, b = 37.61, c = 30.47, β = 112.60 |
| Matthews coefficient VM† (Å3 Da−1) | 2.25 |
| RNA duplexes in asymmetric unit | 1 |
| Solvent content‡ (%) | 58.0 |
| Measured reflections | 133399 |
| Unique reflections | 12058 |
| Resolution range (Å) | 50.0–1.18 (1.22–1.18) |
| Completeness (%) | 93.5 (87.0) |
| Multiplicity | 11.1 (7.5) |
| Rmerge§ (%) | 8.0 (21.3) |
| Average I/σ(I) | 22.9 (1.8) |
Matthews (1968 ▶).
Estimated using the average partial specific volume calculated for RNA by Voss & Gerstein (2005 ▶).
R
merge =
, where I
i(hkl) and 〈I(hkl)〉 are the observed individual and mean intensities of a reflection with indices hkl, respectively,
is the sum over the individual measurements of a reflection with indices hkl and
is the sum over all reflections.