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Molecular-weight determination of cgHle by gel-filtration chromatography. The figure shows an overlay of the chromatogram with a calibration curve obtained using four proteins (ferritin, 440 kDa; catalase, 240 kDa; bovine serum albumin, 66 kDa; cytochrome c, 12.4 kDa). The cgHle peak corresponds to a molecular weight of 77.9 kDa.
