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. 2008 Dec 24;5:163. doi: 10.1186/1743-422X-5-163

Figure 3.

Figure 3

Surface expression of NiV and MV glycoproteins in the presence of increasing amounts of EB2. Vero cells were cotransfected with constant amounts of NiV F- or G-encoding plasmids and the indicated amounts of pCAGGS-EB2. At 24 h p.t., cells were surface biotinylated and lysed. (A) Immunoprecipitation of NiV G was carried out using a polyclonal NiV antiserum. After separation on a 12% SDS gel under reducing conditions and blotting to nitrocellulose, surface-biotinylated G proteins were detected by IRDye 800-conjugated streptavidin using a LiCor-Odyssey imager. (B) NiV F was immunoprecipitated with an F-specific antiserum, separated by SDS-PAGE under non-reducing conditions and further processed as described above. (C) Vero cells were cotransfected with constant amounts of plasmids encoding the MV F and H proteins and the different amounts of pCAGGS-EB2. Immunoprecipitation of the MV glycoproteins was carried out using F- and H-specific antibodies. After separation by SDS-PAGE under reducing conditions and blotting, proteins were detected as described above.