Figure 4.

Identification of differentially expressed genes involved in fatty acid synthesis, beta-oxidation, ketogenesis, gluconeogenesis and cholesterol metabolism. A: Expression of genes present on microarray and analyzed by microarray procedure. Gene symbol: HGNC Hugo abbreviation of the human ortholog of the Gallus gallus gene represented by the oligo spotted on microarray. Results were expressed as a log2 ratio of the gene expression between fasted and fed states ("Fst16-Fed" or "Fst48-Fed" contrasts). Genes in gold were analyzed by qRT-PCR (see B). P values were corrected by Benjamini-Hochberg (see Methods). * pvalue < 0.05, ** pvalue < 0.01, *** pvalue < 0.001, NS: non-significant. B: Validation of microarray results by qRT-PCR. PCRs were realized in triplicate with SyBr Green and specific primers for each gene (See Table 1). 18S ribosomal RNA was used as reference. Results were expressed as a log2 ratio of the gene expression for the two "Fst16-Fed" and "Fst48-Fed" contrasts. Statistical significance is indicated as following: *pvalue < 0.05, **pvalue < 0.01, ***pvalue < 0.001. Genes not analyzed by microarray procedure are indicated with §