Skip to main content
. 2008 Dec 17;9:611. doi: 10.1186/1471-2164-9-611

Table 3.

Selected qRT-PCR primer sequences and accession numbers

Gene symbol Gene name Ensembl or Gene bank Accession number Primer sequence (forward/reverse)
ACACA Acetyl-Coenzyme A carboxylase alpha ENSGALG00000005439 GAGGAGGGAAGGGAATTAGGAA
CCAAGTGGCGGGACTGTT

ACOX1 Acyl-Coenzyme A oxidase 1 ENSGALG00000002159 TCATCCGGTCTCTGATTGTAGGA
GCACTATAGCGGATGGCAATG

ACLY ATP citrate lyase ENSGALG00000003475 GGCGTGAATGAACTGGCTAAC
TAGTCTTGGCATAGTCATAGGTCTGTTG

CPT1A Carnitine palmitoyltransferase1A ENSGALG00000007077 CCCTGAAAATGCTGCTTTCCTA
TGGTGCCTGCAGAAAGTTTG

CPT2 Carnitine palmitoyltransferase II ENSGALG00000010681 CCTGAACGCCCAGAAACCT
CCCTTTTCAAACTGATGAGCAAGT

CYP7A1 Cytochrome P450, family 7, subfamily A, polypeptide 1 ENSGALG00000015432 TGATGACATGGAAAAAGCAAAGA CCAAAAAGTAGCAGGAATGGTGTT

EHHADH Peroxisomal bifunctional enzyme ENSGALG00000006680 TCATAGAAAGGAGCGAGAAGC
AGCAGGAACCCCAACCAGT

FADS1 Fatty acid desaturase 1 ENSGALG00000007127 CAGCACCACGCGAAACC
TCTACAGAGAGCTTCTTTCCCAAAG

FADS2 Fatty acid desaturase 2 ENSGALG00000007178 CCATGATCAAGCGCAGGTT
ATGTATGTGATGAAATAGCGCATGTAG

FASN Fatty acid synthase ENSGALG00000002747 TGAAGGACCTTATCGCATTGC
GCATGGGAAGCATTTTGTTGT

HMGCS1 Hydroxymethylglutaryl-CoA synthase, cytoplasmic ENSGALG00000014862 GCTGGTGCTGTTGCTATGCT
TGTCTGTCCCCTCTTTTTGC

HMGCS2 Hydroxymethylglutaryl-CoA synthase, mitochondrial ENSGALG00000002960 GGTGGTGTGTGGGGACAT
GGTAGCACTGGATGGAGAGG

HMGCR 3-hydroxy-3-methylglutaryl- Coenzyme A reductase ENSGALG00000014948 CTGGGTTTGGTTCTTGTTCA
ATTCGGTCTCTGCTTGTTCA

NR1H3 Nuclear receptor subfamily 1, group H, member 3 (LXRα) ENSGALG00000008202 TCCCACTCAACTCAGCACAC
CAGACTTCATTTCCCAGCATC

PCK1 Phosphoenolpyruvate carboxykinase, cytosolic ENSGALG00000007636 CTGCTGGTGTGCCTCTTGTA
TTCCCTTGGCTGTCTTTCC

PPARA Peroxisome proliferative activated receptor, alpha ENSGALG00000022985 AGCATCCAGTCCTTCATCCA
AAAAACCCTTACAACCTTCACAA

SCD Stearoyl-CoA desaturase(delta-9-desaturase) ENSGALG00000005739 TTTGGCAATCGGCCGTAT
TGGTAGTTGTGGAAACCTTCTCCTA

SREBF1 Sterol regulatory element binding protein 1 gb:AY029224 GTCGGCGATCCTGAGGAA
CTCTTCTGCACGGCCATCTT

SREBF2 Sterol regulatory element binding protein 2 ENSGALG00000011916 GGCTGGCTTCTCCCCCTAT
GTTCATCCTTAACCTTTGCATCAT

Genes in bold were present on the microarray.