Figure 1.

SGC7901/VCR cells that overexpress MDR1 are more resistant to chemotherapy than SGC7901 cells. (A-C) After treatment with different concentrations of 5-FU, cisplatin or epirubicin for 72 hours, cell viability was determined using the MTT assay. The viability of the untreated cells was taken as 100%. Points, mean of three independent experiments; bars, SD. Significant differences are indicated by asterisks. *, P < 0.05. (D) SGC7901/VCR and SGC7901 cells were harvested to prepare cell lysates. The lysates were subjected to SDS-PAGE and blotted with anti-P-gp antibody. (E) RT-PCR assays were performed to detect MDR1 mRNA. A representative example of an experiment that was repeated three times is shown.