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The expression of GRP78 at the protein level by western blots in NCI-H446. (A): untreated (control) cells; (B): cells treated with BAPTA-AM at 10 μM alone for 2 h; (C): cells treated with A23187 at 2 μM alone for 24 h; (D-E): cells treated with BAPTA-AM at 25 μM (D), 40 μM (E) for 2 h respectively, prior to the addition of A23187 at 2 μM for 24 h.I: Electrophoregram II: Bar graph.
