Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Microsc Res Tech. 2009 Feb;72(2):85–92. doi: 10.1002/jemt.20647

Figure 2.

Figure 2

Quantitative morphometry of the z-axis (optical axis) dimension of 3D reconstructed microspheres after structured illumination acquisition. Linescans from a minimim of 5 blue (A), green (D), and red (G) microspheres is shown as raw data (open circles) summarized with a smoothing curve (line) based on a 5 point adjacent average. The diameter of the microsphere, on the basis of manufacturer specifications, is shown (arrows). After empiric software compression (Volocity) to optimize shape factor, the 3D reconstructed microspheres were again measured by z-axis linescan (B,E,H) and summarized by a smoothing curve. The effect of the correction is shown by waterfall comparison of the pre- and post-correction smoothing curves (C,F,I). The structured illumination images of micropheres suspended in glycerol were obtained with 40x oil. immersion objective using selective blue (ex 360/40nm; em 420nm LP), green (ex 480/40nm; ex 535/25nm) and red (ex 540/25nm; em 620/60nm) fluoresence filters.