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Characterization of Palladin knock-out ES cells. A, genotyping of Palladin knock-out ESC by RT-PCR. B, Western blotting confirms Palladin depletion in ESCs. C, palladin knock-out ESCs were stained with ESC markers, Oct4, Sox2, and SSEA-1 to document retention of stem cell pluripotency. D, Palladin is induced during SMC differentiation in wild type mouse ESCs derived from EBs, and the expression of Palladin and SMA was determined by real time RT-PCR at days 10, 15, 20, and 28.
