FIGURE 2.

A, the expression of SMC differentiation marker genes is attenuated in embryoid bodies derived from Palladin knock-out homozygous ESCs. The wild type (WT), heterozygous (Het), and homozygous ESCs (KO) were induced to differentiate into embryoid bodies, and the expression of SMA, SM22, and SM-MHC at day 28 was determined by real time RT-PCR. B, the expression of contractile proteins was reduced in embryoid bodies at day 28. Western blotting shows the expression of SM contractile proteins in the wild type (WT), heterozygous (Het), and homozygous (KO) EBs. Equal amounts of protein were loaded for each. C, results of quantitative analysis of SM contractile proteins. The expression is normalized to GAPDH and the values for Het and KO cells normalized to that of controls.