FIGURE 3.

A, immunofluorescence results in APSCs showing reduced expression of SMA and the focal adhesion protein LPP. Palladin is localized to stress fibers in WT. The diffuse background staining in the Palladin KO is nonspecific. B, Western blotting of APSCs showing decreased MLC20, calponin, and h-caldesmon protein expression in Palladin KO versus WT cells. Subconfluent APSCs were lysed and equal amount of protein were loaded. C, results of quantitative analysis of SM contractile proteins. The expression of each protein is first normalized to GAPDH and then the values for KO cells normalized to that of control cells. D, Western blotting showing decreased SMMHC SM1 and SM2 isoforms and no significant change of non-muscle myosin NMIIA and NMIIB in WT and KO APSCs. Mouse bladder was used as a control for MHC.