FIGURE 6.

A, immunolabeling showing decreased F-actin and increased G-actin in Palladin knock-out APSC. F-actin was identified by phalloidin staining, and G-actin was stained with Alexa Fluor 488-conjugated DNase I in wild type and Palladin knock-out APSCs. B, Western blot analyses showing G:F-actin ratios. The bar graph shows the increase in the G:F-actin ratio in the Palladin null APSCs. C, electron micrographs showing the ultrastructure of APSCs incorporated into collagen gels after 14 days in culture. The WT cells were elongated and in some cases had a basement membrane, whereas the Palladin null cells had a more rounded morphology. BM, basement membrane. F, filament bundles.