FIGURE 5.

Sex-specific increases in lipid droplets and triglycerides during starvation in neurons. A, LipidTox™ staining showing increased aggregation of neutral lipids (green) in neurons from females (XX) during ND. B, lipid aggregates were quantified and expressed as aggregates/cell (identified by Hoechst, blue; n = 5 high power fields/group from three independent experiments). C, typical positive ion ESI-mass spectra of TG molecular species isolated from 10 × 10⁶ pooled primary rat cortical neurons before and after ND and MS/MS of TG major ion at m/z 876.8 (52:2) (D). E, TG content in neurons from males (XY) and females before and after ND (mean ± S.D. from five runs from the pooled samples). Mean ± S.D.; ^*, p < 0.05 versus control; †, p < 0.05 versus XY; ANOVA with Dunnett's post hoc test.