FIGURE 7.

Cytosolic phospholipase A2 (cPLA2)-dependent lipid droplet formation during starvation in neurons. A-C, treatment with the relatively selective cytosolic phospholipase A2 inhibitors AACOCF₃, MAFP, and Py-2 exacerbated ND-induced reductions in mitochondrial respiration as measured by the conversion of MTT to formazan in neurons from females (XX) and to a lesser extent males (XY) versus control at 24 h in a dose-dependent manner (n = 4-8/group). D and E, treatment with AACOCF₃ (100 μm), MAFP (100 μm), and Py-2 (10 μm) inhibited ND-induced formation of neutral lipid droplets detected using LipidTox™ staining (green) in both XY and XX neurons (n = 12 measurements/condition). Mean ± S.D.; †, p < 0.05 versus XY; ‡, p < 0.05 versus vehicle; ANOVA with Dunnett's post hoc test.