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. 2009 Jan 23;284(4):2363–2373. doi: 10.1074/jbc.M806088200

FIGURE 1.

FIGURE 1.

Autophagy is induced by nutrient deprivation in primary cortical neurons. A and B, analysis of GFP-LC3 distribution in primary cortical neurons from GFP-LC3 transgenic mice grown in different culture medium conditions. Primary neurons cultured in CM consisting of Neurobasal-A™ with B27 supplement display diffuse GFP-LC3 staining (A), but primary neurons cultured in NLM with just DMEM show prominent GFP-LC3 puncta formation (B), clearly discernible at higher magnification (arrowheads; see inset). Green, GFP-LC3; blue, DAPI. Scale bar, 20 μm. C, LC3 Western blot analysis of primary cortical neurons grown in CM and NLM. A shift in the LC3 isoform type is progressively observed in primary neurons cultured in NLM. D, quantification of GFP-LC3 puncta formation in primary neurons grown in different culture medium conditions. NLM significantly induces autophagosome formation in comparison with CM (p < 0.05, one-way ANOVA; error bars, S.E.).