FIGURE 8.

Insulin supplementation promotes trAR112Q neurotoxicity. Primary neurons expressing GFP-tagged trAR112Q were cultured for 24 h in either CM or CM supplemented with insulin (CM + INS). When we quantified caspase-3 activation, we noted a significant increase in trAR112Q-expressing neurons cultured in CM plus insulin. Increased AR112Q neurotoxicity in primary cortical neurons cultured in CM plus insulin was accompanied by decreased MAP2 staining and increased nuclear condensation (not shown).