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. 2009 Jan 23;284(4):2064–2071. doi: 10.1074/jbc.M804292200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — MTMR6 co-localizes with MTMR9. A, HeLa cells were transfected with MTMR6-HA, MTMR9-FLAG, or both. After fixation, the cells were co-stained with anti-HA (rabbit) antibody and anti-FLAG monoclonal antibody conjugated with cy3 followed by Alexa Fluor 488-anti-rabbit antibody. The co-localization images are shown at high magnification (×100). 4′,6-Diamidino-2-phenylindole (DAPI) staining shows nuclei. B, HeLa cells were transfected with MTMR6-HA or MTMR9-FLAG. After fixation, the cells were co-stained with anti-HA (rabbit) polyclonal antibody (MTMR6 panel) or anti-FLAG (rabbit) polyclonal antibody (MTMR9 panel) and anti-calnexin antibody (mouse) monoclonal antibody followed by Alexa Fluor 488-anti-rabbit antibody and Alexa Fluor 555-anti-mouse antibody.