FIGURE 5.

p120ctn associates with γ-secretase and links it to cadherins. Extracts prepared in 0.2% dodecyl maltoside from HEK293 cell cultures were immunoprecipitated with antibodies against APH-1, NCT, PS1-CTF, PS1/NTF, and p120ctn. Obtained IPs were separated by SDS-PAGE and probed on WBs for antigens indicated at the right of the figure. Note that each γ-secretase component co-immunoprecipitates with p120ctn. Conversely, this catenin co-immunoprecipitates with each γ-secretase component probed. Mature full-length APP (APP FL)interacts specifically with nicastrin, whereas N-cadherin interacts with p120ctn. Nonimmune serum (Ab ctrl) and beads alone were used as controls.