FIGURE 11.

Kinetics of formation of the functional NS3-RNA complex measured with a 34-bp RNA substrate with varying lengths of the single-stranded 3′-tail: 6 nt (triangles), 10 nt (squares), and 20 nt (circles). RNA concentration was 25 nm (green symbols and lines), 50 nm (red symbols and lines), and 100 nm (black symbols and lines). NS3 concentration was 50 nm in all experiments. Low salt conditions are shown (0 mm NaCl); experiments in the presence of 30 mm NaCl produced similar results (data not shown).