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. 2009 Jan 23;284(4):2512–2521. doi: 10.1074/jbc.M805460200

FIGURE 5.

FIGURE 5.

The apparent kinetic step size of RNA unwinding by NS3 is sensitive to monovalent salt concentration. A, numbers of kinetic steps determined for the substrate FLAT1 at 0, 30, and 60 mm NaCl (in addition to 20 mm MOPS-NaOH, pH 6.5) plotted versus RNS duplex length. B, apparent step size of NS3 unwinding at varying NaCl concentrations determined from the slopes of linear fits from a. Except for 100 mm NaCl, duplicate experiments are shown.