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. 2009 Jan 23;284(4):1982–1989. doi: 10.1074/jbc.M807651200

FIGURE 4.

FIGURE 4.

G9a in the liver and cortex. A, RNA was isolated from the liver of E17 embryos and used for RT-PCR of G9a and normalized to β-actin (see example in gel insets). B, G9A protein levels in E17 liver were analyzed by immunoblot and normalized to β-ACTIN (see example in blot insets). C, RNA was isolated from the cortex of E17 embryos and used for RT-PCR of G9a and normalized to β-actin (see example in gel insets). D, G9A protein levels in E17 cortex were analyzed by immunoblot and normalized to the levels ofβ-ACTIN (see example in blot insets). See “Results” for statistics. Bar labels: S, choline-supplemented; C, controls; D, choline-deficient.