Suv39h1 in the liver and cortex. A, RNA was isolated from
the liver of E17 embryos and used for RT-PCR of Suv39h1 and
normalized to β-actin (see example in gel insets).
B, SUV39H1 protein levels in E17 liver were analyzed by immunoblot
and normalized to β-ACTIN (see example in blot insets).
C, RNA was isolated from the cortex of E17 embryos and used for
RT-PCR of Suv39h1 and normalized to β-actin (see
example in gel insets). D, SUV39H1 protein levels in E17
cortex were analyzed by immunoblot and normalized to the levels of
β-ACTIN (see example in blot insets). See “Results”
for statistics. Bar labels: S, choline-supplemented; C,
controls; D, choline-deficient.