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Methylation-specific PCR (MSP) analysis of G9a and Suv39h1 promoter CpG islands. A–C, genomic DNA isolated from E17 liver (A and B) or cortex (C) was treated with sodium bisulfite and analyzed by methylation-specific PCR. The inset shows examples of the PCR products obtained using the primers designed to amplify the methylated (m) and unmethylated (u) DNA templates. See “Results” for statistics. Bar and PCR product labels: S, choline-supplemented; C, controls; D, choline-deficient.
