TABLE 2.
Kinetic parameters of the SorAB sulfite dehydrogenase (SDHWT) from S. novella
| pH | Km(sulfite) | kcat | kcat/Km(sulfite) |
|---|---|---|---|
| mm | s-1 | m-1 s-1 | |
| 6.0 | 0.6 × 10-3 ± 9.5 × 10-5 | 63.5 ± 2.2 | 1.06 × 108 |
| 6.5 | 1.1 × 10-3 ± 0.14 × 10-3 | 86.2 ± 2.2 | 7.55 × 107 |
| 7.0 | 3.7 × 10-3 ± 0.45 × 10-3 | 158.8 ± 4.5 | 4.27 × 107 |
| 7.5 | 7.1 × 10-3 ± 0.82 × 10-3 | 293.4 ± 7 | 4.03 × 107 |
| 8.0 | 2.2 × 10-2 ± 0.26 × 10-2 | 345.3 ± 11 | 1.53 × 107 |
| 8.5 | 8.6 × 10-2 ± 0.85 × 10-2 | 410 ± 11 | 4.88 × 106 |
| 9.0 | 0.324 ± 0.029 | 519 ± 11 | 1.5 × 106 |
| 9.5 | 1.66 ± 0.18 | 431 ± 16 | 2.51 × 105 |
| 10.0 | 3.389 ± 0.44 | 23.7 ± 1.3 | 6.77 × 103 |