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. 2009 Jan 23;284(4):2307–2319. doi: 10.1074/jbc.M806434200

FIGURE 3.

FIGURE 3.

Growth of PRC shRNA transductants and controls on either glucose or galactose as the primary carbon source. A, growth of U2OS wild type (wt; open squares) and lentiviral transductants expressing either the control shRNA (control; closed squares), PRC shRNA1(sh1; closed circles), or PRC shRNA4 (sh4; open circles) on glucose media. B, flow cytometric DNA content analysis of control shRNA, PRC shRNA1, and PRC shRNA4 U2OS transductants stained with propidium iodide. Representative DNA histograms are shown. Quantitative assessment of the cell cycle distributions are shown in each panel. Values are the averages ± S.E. for three independent determinations. C, growth of U2OS wild type (open squares) and lentiviral transductants expressing either the control shRNA (closed squares), PRC shRNA1 (closed circles), or PRC shRNA4 (open circles) on galactose media. Growth curves in A and B represent the averages ± S.E. for three separate determinations.