Growth of PRC shRNA transductants and controls on either glucose or
galactose as the primary carbon source. A, growth of U2OS wild
type (wt; open squares) and lentiviral transductants
expressing either the control shRNA (control; closed
squares), PRC shRNA1(sh1; closed circles), or PRC
shRNA4 (sh4; open circles) on glucose media. B,
flow cytometric DNA content analysis of control shRNA, PRC shRNA1, and PRC
shRNA4 U2OS transductants stained with propidium iodide. Representative DNA
histograms are shown. Quantitative assessment of the cell cycle distributions
are shown in each panel. Values are the averages ± S.E. for
three independent determinations. C, growth of U2OS wild type
(open squares) and lentiviral transductants expressing either the
control shRNA (closed squares), PRC shRNA1 (closed circles),
or PRC shRNA4 (open circles) on galactose media. Growth
curves in A and B represent the averages ± S.E.
for three separate determinations.