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. 2009 Feb 6;5(2):e1000288. doi: 10.1371/journal.ppat.1000288

Figure 4. Host cells die after disruption of the PVM and death of the T. gondii.

Figure 4

MEFs were treated with 200 U/ml IFNγ and co-transfected with Irga6-ctag1-EGFP and pDsRed constructs for 24 hours. Cells were then infected with ME49 strain T. gondii at MOI between 5 and 10 for 1 hour and observed microscopically by time-lapse photography. Three PVs were observed within this cell when the experiment started. Images were taken at 5 minutes intervals, and the complete series is shown. Two Irga6-ctag1-EGFP-positive PVMs were disrupted at 1:10 and 1:40, respectively (arrows). These two T. gondii became permeable to mDsRed at 1:30 and 2:25, respectively (arrowheads). At 2:30 after infection, the mDsRed signal suddenly disappeared from the host cell accompanied by a drastic change in host cell morphology shown in phase contrast images. Scale bar: 10 µm. The videos from which these frames were extracted are presented as Videos S9 and S10.