Fig. 2.

nkd is a TAF4-dependent gene in larval wing imaginal discs. (A) TAF4 RNAi in larval imaginal wing discs reduced expression of nkd. (Upper) Wg expression. (Lower) Endogenous nkd staining in discs expressing either GFP, an Arm-inverted repeat, or a TAF4-inverted repeat in a perpendicular pattern. Arrowhead indicates intersection between endogenous nkd expression and dpp-Gal4 driven expression of the indicated constructs. (Scale bars: 100 μm.) (B) TAF4 and RNAP II occupancy of the nkd promoter was analyzed by ChIP in cells treated with either buffer or Wg for 2 h. Immunoprecipitated chromatin was quantified by qRT-PCR relative to control serum.