Fig. 3.

The TAF4 CTR does not rescue TAF4 RNAi for nkd activation. (A) Stable cell lines expressing either full-length TAF4 (TAF4 FL) or the TAF4 CTR were treated with dsRNA targeting the TAF4 UTRs. The cells were split and transfected with either empty vector or Arm* to activate nkd transcription. The levels of nkd were analyzed as before. (B) Transfected Arm* levels were equivalent in TAF4FL and TAF4 CTR cell lines as determined by α-V5 immunoblotting. Endogenous TAF4 levels were depleted efficiently in both cells. β-Tubulin was included as a loading control. (C) Overexpression of the TAF4 CTR, but not full-length TAF4, reduced nkd expression when coexpressed with Arm*. (D) Transfected Arm* levels were equivalent as determined by immunoblotting with the α-V5 antibody. β-Tubulin served as a loading control.