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. 2008 Dec 30;106(1):55–60. doi: 10.1073/pnas.0811914106

Fig. 5.

Fig. 5.

The ETO domain interacts with Pygopus in a GST pull-down experiment. (A) (Left) A schematic representation of the GST fusion protein used in the pull-down experiments. (Right) A Coomassie-stained gel loaded with protein molecular weight markers (M), GST, or GST-ETO beads. (B) GST pull-down experiment using either GST or GST-ETO beads incubated with lysates made from S2R+ cells transfected with V5 tagged Arm*, Pangolin, or Pygopus. Bound proteins were analyzed by α-V5 immunoblotting. (C) GST pull-down experiment performed as in B using lysates made from S2R+ cells transfected with V5 tagged Pygopus constructs encoding either residues 1–200 or 201–815. (D) GST pull-down experiment performed as in B using lysates made from S2R+ cells transfected with either wild-type Pygo 1–200 (wt) or a Pygo 1–200 construct with the NPFxD motif mutated to AAAxA (mut). (E) GST pull-down experiment performed as in B using lysates made from bacteria transformed with a plasmid encoding V5 tagged Pygo 1–200.