Fig. 3.

Yeast rolling profiles. (A and B) Cumulative distance rolled of tracked cells at 4 dyn/cm² for wild-type P-selectin and A28H P-selectin, respectively. Each line represents a single cell. (C) Average rolling velocity ± SD, calculated from the average rolling velocities determined from each of 9 separate trials. See Materials and Methods for more detail. (D) Average number of cells tracked at each shear stress. Densities of cells infused to the flow chamber were comparable. (E) Quantitation of rolling stability. Of the 6 seconds tracked at each shear, percentage of time that the instantaneous velocity was zero. (F) Another measure of rolling stability. Of the 6 s tracked, percentage of time that cells were adherent (rolling or stopped but attached) as opposed to detaching and no longer being tracked in the 6-s window.