Fig. 1.

Inactivation of Gtf2ird1 and Gtf2i using the gene-trap strategy (http:/www.genetrap.org/). Mutant mice were produced from gene-trap lines carrying the β-Geo insertion within intron 22 (clone Gtf2ird1^XE465) or intron 3 (Gtf2i^XE029). Geo, lacZ-neo insertion; NLS, nuclear localization signal. Animals were genotyped by PCR with primers specific for Gtf2i (TF2IF2/TF2IR2 and XE029F/XE029R1) and Gtf2ird1 (F11/BEN22R/XF06). The WT Gtf2i allele gives a 437-bp band (line 1), whereas the Gtf2i^XE029 allele displays a 200-bp product (line 2). Similarly, Gtf2ird1-specific primers amplify a 479-bp fragment in WT DNA (line 3) and an additional 320-bp band from the Gtf2ird1^XE465 gene-trapped allele (line 4). Positions of primers used in PCR are shown in the diagram.