Fig. 6.

TFII-I transcription factors regulate TGFβRII/Alk1/Smad5 and Vegfr2 signal transduction cascades. Changes in expression of genes involved in early vasculogenesis and angiogenesis in Gtf2i and Gtf2ird1 KO embryos are based on microarray data, Gtf2i and Gtf2ird1 knockdown data were obtained by quantitative (q) RT-PCR after posttranscriptional silencing of Gtf2i or Gtf2ird1 in ES cells by gene-specific siRNAs, and Gtf2ird1 overexpression results are based on qRT-PCR data obtained after doxycycline induction of Gtf2ird1 in a Tet-on ES cell line. Error bars represent the SD calculated from 3 or 2 biological repeats.