Fig. 3.

PKCη directly interacts with occludin. (A and B) MDCK cells expressing PKCη_WT-GFP were fixed and double stained for occludin and GFP. The x–y (A) and x–z (B) fluorescence images were collected. (C and D) Anti-occludin immunocomplexes (nondenatured) from cells transfected with PKCη_WT-GFP or vector were immunoblotted for occludin and GFP (C). Similarly, GFP was immunoprecipitated and immunoblotted for GFP and occludin (D). (E) Recombinant proteins GST or GST-Occludin-C were incubated with recombinant PKCη. GST and GST-Occludin-C were pulled down with GSH-agarose and immunoblotted for PKCη and GST. (F) Densitometric analysis of PKCη bands in experiment described in E. Values are mean ± SEM (n = 3).