Fig. 4.

Cell death and disease-resistance phenotypes of incompatible lines. (A) Spontaneous cell death revealed by TB staining of 3-week-old leaves of incompatible lines (4, NIL) and parental accessions grown at 14 °C (Left) or 20 °C (Right). (B) Infection phenotypes of the same lines as in (A). Two-week-old plants grown at 14 °C (Left) or 20 °C (Right) were inoculated with virulent H. parasitica isolate Cala2 at 18 °C. Microscopic examination of TB-stained leaves to reveal dead plant cells and pathogen mycelium growth was performed 4 days postinoculation. (Scale bars, 500 μm.)