Fig. 5.

The distribution of unmodified and methylated histones in infected and transfected/infected cells. HEp-2 cells were either mock infected, infected, or transfected/infected as in Fig. 4, except that the cells were fixed at 4h after infection. The mock infected cells were reacted with the mouse monoclonal antibody to PML and the rabbit polyclonal antibodies to histone H3, tri-methyl H3K9, tri-methyl H3K27, or tri-methyl H4K20. The infected or transfected/infected cells were reacted with the mouse monoclonal antibody to ICP0 and the histones antibodies, described above for mock. The images were captured as in Fig. 4.