Fig. 2.

RNA polymerase II recruitment to transcribed globin genes is reduced by HS5 in a CTCF-dependent manner but recruitment to the LCR is unaffected. ChIP was carried out with antibodies to RNA pol II or control IgG and anemic spleen chromatin. Real-time qPCR was carried out with primers and TaqMan probes recognizing (A) individual globin genes or (B) LCR HS sites. For sequences of primers and TaqMan probes, see Tables S2 and S3. Note that the HS5 primers and probe recognize both the endogenous and ectopic copies of HS5. The mouse actin signal was used to normalize results between experiments. Error bars represent SEM and Student's t Test indicates P < 0.02, when comparing the adult globin promoters in WT versus HS5 mice.