Fig. 3.

Histone H3 and H4 acetylation at adult δ- and β-globin genes is reduced by ectopic HS5 in a CTCF-dependent manner, while acetylation across the LCR is not reduced. ChIP assays and real-time qPCR analysis was carried out as described in the legend to Fig. 2, using antibodies against (A) di-acetylated histone H3 and (B) tetra-acetylated H4. Note that the eHS5 primer recognizes the unique junction between ectopic HS5 and sequences upstream of ε-globin. The results were normalized to the mouse actin signal and are shown ± SEM. P < 0.04 when comparing the adult globin promoters in WT versus HS5 mice.