Fig. 4.

ZicA and AP-2 act in parallel pathways to bring about the formation of the neural plate border. Embryos were injected with AP-2 (A) or ZicA (C) MO or a combination of ZicA MO and AP-2 mRNA (B) or AP-2 MO and ZicA mRNA (D). The effects on neural crest formation were assessed at E5.5 by staining for the neural crest marker FoxD-A. Both AP-2 and ZicA MO abolish the marker expression on the injected side (A and C), whereas coinjection with ZicA mRNA and AP-2 mRNA, respectively, does not rescue this phenotype (B and D). These experiments suggest that these 2 genes act in parallel pathways. Black lines indicate the midline. (Magnification: 40×.)