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. 2008 Dec 17;105(51):20309–20314. doi: 10.1073/pnas.0805690105

Fig. 2.

Fig. 2.

Skeletal growth retardation caused by impaired growth plate chondrocyte proliferation. (A) Representative micrographs of H&E stained sections of the proximal ends of tibiae from E18.5 wild-type (WT) and Pthrp KI mice (magnification, ×100). Blue lines represent the respective proliferating zones. (B) The length measurements of the proliferating zone. (C) Representative contact radiographs of the femurs of WT and Pthrp KI mice at 2 weeks of age. (D) Femoral length measurements. (E) BMD measurements. (F) Representative frontal views of microCT 3D reconstruction of the proximal end of tibiae. (G) Epiphyseal volume of the proximal ends of tibiae. (H) Width of the cartilaginous growth plate. (I) Undecalcified sections of tibiae stained by the von Kossa procedure (magnification, ×200). (J) Mineralized area, percentage of growth plate. (K) Paraffin-embedded sections of tibiae from WT and KI mice stained immunohistochemically for PCNA (arrowheads) (magnification, ×400). (L) Number of PCNA-positive chondrocytes as a percentage of total chondrocytes, as determined by image analysis. (M) Immunostaining for type X collagen (arrowheads) (magnification, ×100). (N) Width of type X collagen-positive hypertrophic zone of growth plates. Data shown represent mean ± SE of five animals per group. **, P < 0.01; ***, P < 0.001 in the KI mice relative to the wild-type mice.