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Polyfunctional NY-ESO-1 antigen-specific T cells secreted higher levels of IFN-γ after anti-CTLA-4 antibody treatment. (A) IFN-γ fluorescence of CD4+ (Top) and CD8+ (Bottom) T cells with different functional properties before and after anti-CTLA-4 antibody treatment. Representative example from patient IMF-8. (B) Compiled IFN-γ MFI of CD4+ T cells of different cytokine combinations.
