Table 2.
Activity of HesC reporter constructs
Reporter | No. injected | WT pattern*/total GFP+ | Zone 4 | Strong zone 3 |
---|---|---|---|---|
HesC BAC | 223 | 70/89 | 0/89 | 10/89 |
10 kb reporter | 160 | 45/56 | 0/56 | 8/56 |
w/Δ Blimp site | 127 | 0/36 | 31/36 | 24/36 |
w/Δ Su(H) site | 119 | 0/32 | N/D | N/D |
*Wild-type reporter activity scored as pattern of fluorescence seen in BAC and 10 kb constructs (Fig. 5A) at 27 h postfertilization—namely, weak activity in zone 1, strong activity zone 2, intermediate activity zone 3 (NSM), and no activity zone 4 (ingressed skeletogenic mesoderm).