Table 1.
Particle | AIII, % | DIII, μm2/s | AII, % | DII, μm2/s | AI, % | DI, μm2/s | n |
---|---|---|---|---|---|---|---|
DNA-labeled BR mRNP | 22 ± 9 | 0.24 ± 0.05 | 65 ± 7 | 0.7 ± 0.1 | 14 ± 4 | 4.0 ± 1.28 | 49,547 |
Qdots 655 | 40 ± 8 | 1.43 ± 0.15 | 60 ± 7 | 4.17 ± 0.39 | 3,598 | ||
Beads (210 nm) | 4 ± 0.9 | 0.01 ± 0.002 | 30 ± 11 | 0.13 ± 0.03 | 66 ± 11 | 0.4 ± 0.05 | 18,743 |
FITC-dextran,* 500 kDa | 100 | 2.42 ± 0.2 |
A, relative fraction of total absorbance; n, number of single jumps from frame to frame.
*This diffusion constant was determined by a FRAP experiment; see SI Materials.